The anti-inflammatory, anti-oxidant and protective effects of probiotic mixture on organ toxicity in a rat model
Authors:
Murat Karamese, Hakan Aydin, Volkan Gelen, Emin Sengul & Selina Aksak Karamese
Abstract:
The objective of this study was to evaluate the possible protective effects of probiotic bacteria, especially Bifidobacterium and Lactobacillus strains, on 4,4 -dichlorodiphenyltrichloroethane (DDT)-induced toxicity. For this reason, we evaluated the relationship between probiotics and toxicity by checking immunological and immunohistochemical parameters.
Materials & methods: Probiotic pretreatment was applied to 36 Wistar albino rats for 12 consecutive days. Serum aspartate aminotransferase and alanine aminotransferase levels were detected CD3 and NF-κB staining methods were then performed by immunohistochemistry. Finally, pro- and anti-inflammatory cytokines were measured by ELISA.
Results: DDT caused a serious increase/decrease in some cytokine parameters. The effective dose was 1 × 1011 colony-forming unit probiotic treatment. CD3 and NF-κB positivity were intense in DDT group whereas the intensity was reduced in probiotic treatment groups.
Discussion: The probiotic mixture has a potential to prevent inflammatory and oxidative stress-related organ injuries. Further studies should be performed to explain the possible mechanisms.
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